Device

Part:BBa_K2277555

Designed by: Geyi Wang   Group: iGEM17_ZJUT-China   (2017-10-24)


A blue light sensor and an eGFP reporter which it negatively regulates

This is a blue light sensor and its eGFP reporter. The eGFP gene will express In the absence of blue light. The blue-light-sensitive system can be reversed by adding cI repressor protein. The sensor is almost as same as Part:BBa_K1026010 except that FixK2 promoter's sequence is different. This part is the improvement of Part:BBa_K1065303:https://parts.igem.org/Part:BBa_K1065303 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


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